Ranges with the greatest evidence of transcription factor binding for the NR3C1 transcription factor from ENCODE (DDC accession: ENCFF002CFS). For demonstration purpose.

Ranges representing the greatest evidence of enrichment for the NR3C1 transcription factor (DCC accession: ENCFF002CFS) for regions chr2:40000000-50000000 and chr3:10000000-13000000 from the Encyclopedia of DNA Elements (ENCODE) data (Dunham I et al. 2012).

data(A549_NR3C1_CFS_NarrowPeaks_partial)

Format

A GRanges containing one entry per site. The ranges are surronding the peaks present in the dataset A549_NR3C1_CFs_Peaks_partial.

Details

The peaks and ranges have been obtained using an optimal IDR analysis done on all replicates.

References

• Dunham I, Kundaje A, Aldred SF, et al. An integrated encyclopedia of DNA elements in the human genome. Nature. 2012 Sep 6;489(7414):57-74.

See also

• A549_NR3C1_CFS_Peaks_partial the associate genomic regions dataset.

• findConsensusPeakRegions for extracting regions sharing the same features in more than one experiment.

Examples

## Loading datasets
data(A549_NR3C1_CFQ_NarrowPeaks_partial)
data(A549_NR3C1_CFQ_Peaks_partial)
data(A549_NR3C1_CFS_NarrowPeaks_partial)
data(A549_NR3C1_CFS_Peaks_partial)

## Assigning experiment name to each row of the dataset.
## NarrowPeak and Peak datasets from the same experiment must
## have identical names.
names(A549_NR3C1_CFQ_NarrowPeaks_partial) <- rep("NR3C1_CFQ",
                            length(A549_NR3C1_CFQ_NarrowPeaks_partial))
names(A549_NR3C1_CFQ_Peaks_partial) <- rep("NR3C1_CFQ",
                            length(A549_NR3C1_CFQ_Peaks_partial))
names(A549_NR3C1_CFS_NarrowPeaks_partial) <-rep("NR3C1_CFS",
                            length(A549_NR3C1_CFS_NarrowPeaks_partial))
names(A549_NR3C1_CFS_Peaks_partial) <- rep("NR3C1_CFS",
                            length(A549_NR3C1_CFS_Peaks_partial))

## Calculating consensus regions for chromosome 2
## with a default region size of 400 bp (2 * extendingSize).
## The consensus regions are not resized to fit the narrowPeak regions.
## Peaks from both experiments must be present in a region to
## be retained as a consensus region.
chrList <- Seqinfo(c("chr2"), c(243199373), NA)
findConsensusPeakRegions(
    narrowPeaks = c(A549_NR3C1_CFQ_NarrowPeaks_partial,
                            A549_NR3C1_CFS_NarrowPeaks_partial),
    peaks = c(A549_NR3C1_CFQ_Peaks_partial,
                            A549_NR3C1_CFS_Peaks_partial),
    chrInfo = chrList,
    extendingSize = 200,
    expandToFitPeakRegion = FALSE,
    shrinkToFitPeakRegion = FALSE,
    minNbrExp = 2,
    nbrThreads = 1)
#> $call
#> findConsensusPeakRegions(narrowPeaks = c(A549_NR3C1_CFQ_NarrowPeaks_partial, 
#>     A549_NR3C1_CFS_NarrowPeaks_partial), peaks = c(A549_NR3C1_CFQ_Peaks_partial, 
#>     A549_NR3C1_CFS_Peaks_partial), chrInfo = chrList, extendingSize = 200, 
#>     expandToFitPeakRegion = FALSE, shrinkToFitPeakRegion = FALSE, 
#>     minNbrExp = 2, nbrThreads = 1)
#> 
#> $consensusRanges
#> GRanges object with 8 ranges and 0 metadata columns:
#>       seqnames            ranges strand
#>          <Rle>         <IRanges>  <Rle>
#>   [1]     chr2 42291696-42292096      *
#>   [2]     chr2 44518668-44519068      *
#>   [3]     chr2 46049016-46049416      *
#>   [4]     chr2 46542378-46542778      *
#>   [5]     chr2 46875290-46875690      *
#>   [6]     chr2 47082519-47082919      *
#>   [7]     chr2 47194248-47194648      *
#>   [8]     chr2 47418971-47419371      *
#>   -------
#>   seqinfo: 1 sequence from an unspecified genome; no seqlengths
#> 
#> attr(,"class")
#> [1] "consensusRanges"